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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in lots of tumors, which results in chromosome instability, and therefore may contribute to tumorigenesis. This protein was observed to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant purpose in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two more splice variants had been claimed but specific nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind exclusively to the target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.
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This guidebook outlines further doable optimization of cellular and immunoassay parameters to be certain the absolute best success are...
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This guide outlines further more doable optimization of cellular and immunoassay parameters to be certain the absolute best outcomes are...
Antibodies sure to the blocking peptide not bind to the epitope about the target protein. By evaluating the staining in the blocked antibody as opposed to the antibody alone, you can see which staining is specific.
Focus on Information Cyclin E belongs on the highly conserved cyclin household, whose users are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinctive expression and degradation patterns which lead to your temporal coordination of each mitotic occasion. Cyclin E kinds a posh with and capabilities as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and it is degraded as cells progress by way of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation click here of receptor activation by each antibodies...
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